What do I mean by "aiming up"? Academic journals are ranked by how frequently they get cited in other literature. The more-cited journals (the cream of the crop being Science and Nature) are more prestigious, reach a broader audience, and are much more difficult to get a manuscript accepted. Their impact factors are around 30. For comparison, the first place we submitted had an impact factor 1-1.5. Then we submitted it to a journal with impact factor 0.5-1. This isn't world-changing science, but it's something that other folks who are interested in these things would want to know.
Sam really thinks this manuscript has a shot at a >4 impact factor journal, so that's where we're submitting next. The paper has changed and grown a lot, but I'm still anxious about sending it there, because I'm pretty sure it's going to get rejected without review. If that happens, I think the next place to send it is down... way down (someplace that doesn't even have an impact factor). I guess it could go to another ~1 journal, but I'm skeptical there too.
I just turned over another draft to Sam to work his magic on it because he's the one with the vision and confidence to aim high... fingers crossed. I want this thing accepted somewhere this summer.